Workflow

1 Prepare biological sample, e.g. cheek cells

2 Extract mitochondrial DNA

3 Perform PCR for clade specific region of most abundant haplotype

4 Prepare agarosegel

5 Test for successful PCR

6 Perform REnzyme action on PCR products

7 Evaluate complete REnzyme digest and analyse digest fragments

8 Assign haplotype 

or repeat from step 3 with next most abundant haplotype


alternative workflow:

1 ...

2 ...

3 Perform PCR for HVR1 a/o HVR2- region

4 ...

5 ...

6 Estimate DNA-concentration

7 Submit to sequencing

8 Assign haplotype

made by MerLiN 2008-1010  best with Safari at 1440x900